Microsatellite instability in poorly differentiated adenocarcinomas of the colon and rectum: relationship to clinicopathological features.

ABSTRACT:

Background

Poorly differentiated adenocarcinomas of the colon and rectum (Por) feature the worst prognosis among the various types of colorectal carcinomas. Por is highly associated with microsatellite instability (MSI), although MSI is associated with an improved prognosis in colorectal cancers.

Aim

To investigate the influence of MSI on clinicopathological features and survival of patients affected by Por.

Methods

53 patients affected by Por were investigated. DNA extracted from tumour sections and the corresponding normal tissue was analysed by PCR at five microsatellite loci: BAT25, BAT26, D2S123, D5S346 and D17S250. Tumours with alterations at two or more loci were classified as MSI-Por. The others were classified as microsatellite stability (MSS)-Por. The clinic

SUBMITTER: Kazama Y 

PROVIDER: S-EPMC1955052 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature