Genetic variations of Toll-like receptor 9 predispose to systemic lupus erythematosus in Japanese population.

ABSTRACT:

Background

Systemic lupus erythematosus (SLE) is characterised by dysregulation of autoreactive lymphocytes and antigen-presenting cells. Signalling through Toll-like receptor 9 (TLR9), a mediator of innate immune responses, has a role in activation of dendritic cells and autoreactive B cells.

Objective

To investigate whether TLR9 polymorphisms are associated with an increased risk of SLE.

Methods

DNA samples were obtained from 220 Japanese patients with SLE (with >4 American College of Rheumatology criteria for SLE) and 203 controls. The genetic variations of TLR9 were detected by PCR, followed by DNA sequencing. The promoter and enhancer activities of TLR9 were measured by luciferase reporter gene assay. The titres of anti-dsDNA antibodies in sera from control or TL

SUBMITTER: Tao K 

PROVIDER: S-EPMC1955115 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature