MicroRNA expression dynamics during murine and human erythroid differentiation.

ABSTRACT:

Objective

MicroRNAs (miRNAs) are an abundant class of small noncoding RNAs that regulate diverse cellular functions by sequence-specific inhibition of gene expression. We determined miRNA expression profile during erythroid differentiation and putative roles in erythroid differentiation.

Methods

The expression profile of 295 miRNAs before and after their erythroid differentiation induction was analyzed using microarray. Fluorescein-activated cell sorting analysis was used to isolate mouse spleen erythroblasts at different differentiation stages. Human cord blood CD34+ progenitors were differentiated in vitro. Real-time reverse transcriptase polymerase chain reaction was used to confirm the results of miRNA microarray. Synthetic oligonucleotides for miR-451 overexpression or

SUBMITTER: Zhan M 

PROVIDER: S-EPMC1955220 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature