Integrative characterization of germ cell-specific genes from mouse spermatocyte UniGene library.

ABSTRACT:

Background

The primary regulator of spermatogenesis, a highly ordered and tightly regulated developmental process, is an intrinsic genetic program involving male germ cell-specific genes.

Results

We analyzed the mouse spermatocyte UniGene library containing 2155 gene-oriented transcript clusters. We predict that 11% of these genes are testis-specific and systematically identified 24 authentic genes specifically and abundantly expressed in the testis via in silico and in vitro approaches. Northern blot analysis disclosed various transcript characteristics, such as expression level, size and the presence of isoform. Expression analysis revealed developmentally regulated and stage-specific expression patterns in all of the genes. We further analyzed the genes at the protein and

SUBMITTER: Choi E 

PROVIDER: S-EPMC1955454 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature