Microfluidic system for on-chip high-throughput whole-animal sorting and screening at subcellular resolution.

ABSTRACT: We report a suite of key microfluidic devices for complex high-throughput whole-animal genetic and drug screens. We demonstrate a high-speed microfluidic sorter that can isolate and immobilize Caenorhabditis elegans in a well defined geometry for screening phenotypic features at subcellular resolution in physiologically active animals. We show an integrated chip containing individually addressable screening-chamber devices for incubation and exposure of individual animals to biochemical compounds and high-resolution time-lapse imaging of many animals on a single chip without the need for anesthesia. We describe a design for delivery of compound libraries in standard multiwell plates to microfluidic devices and also for rapid dispensing of screened animals into multiwell plates. When used i

SUBMITTER: Rohde CB 

PROVIDER: S-EPMC1955819 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature