Liposome-mediated cellular delivery of active gp91(phox).

ABSTRACT:

Background

Gp91(phox) is a transmembrane protein and the catalytic core of the NADPH oxidase complex of neutrophils. Lack of this protein causes chronic granulomatous disease (CGD), a rare genetic disorder characterized by severe and recurrent infections due to the incapacity of phagocytes to kill microorganisms.

Methodology

Here we optimize a prokaryotic cell-free expression system to produce integral mammalian membrane proteins.

Conclusions

Using this system, we over-express truncated forms of the gp91(phox) protein under soluble form in the presence of detergents or lipids resulting in active proteins with a "native-like" conformation. All the proteins exhibit diaphorase activity in the presence of cytosolic factors (p67(phox), p47(phox), p40(phox) and Rac) and ara

SUBMITTER: Marques B 

PROVIDER: S-EPMC1955831 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature