Establishment and mitotic stability of an extra-chromosomal mammalian replicon.

ABSTRACT:

Background

Basic functions of the eukaryotic nucleus, like transcription and replication, are regulated in a hierarchic fashion. It is assumed that epigenetic factors influence the efficiency and precision of these processes. In order to uncouple local and long-range epigenetic features we used an extra-chromosomal replicon to study the requirements for replication and segregation and compared its behavior to that of its integrated counterpart.

Results

The autonomous replicon replicates in all eukaryotic cells and is stably maintained in the absence of selection but, as other extra-chromosomal replicons, its establishment is very inefficient. We now show that following establishment the vector is stably associated with nuclear compartments involved in gene expression and chr

SUBMITTER: Stehle IM 

PROVIDER: S-EPMC1959191 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature