Systematic identification of cis-regulatory sequences active in mouse and human embryonic stem cells.

ABSTRACT: Understanding the transcriptional regulation of pluripotent cells is of fundamental interest and will greatly inform efforts aimed at directing differentiation of embryonic stem (ES) cells or reprogramming somatic cells. We first analyzed the transcriptional profiles of mouse ES cells and primordial germ cells and identified genes upregulated in pluripotent cells both in vitro and in vivo. These genes are enriched for roles in transcription, chromatin remodeling, cell cycle, and DNA repair. We developed a novel computational algorithm, CompMoby, which combines analyses of sequences both aligned and non-aligned between different genomes with a probabilistic segmentation model to systematically predict short DNA motifs that regulate gene expression. CompMoby was used to identify conserved ov

SUBMITTER: Grskovic M 

PROVIDER: S-EPMC1959362 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature