Characterization of predominant bacteria isolates from clean rooms in a pharmaceutical production unit.

ABSTRACT:

Aims

To screen for the predominant bacteria strains distributed in clean rooms and to analyze their phylogenetic relationships.

Methods and results

The bacteria distributed in air, surfaces and personnel in clean rooms were routinely monitored using agar plates. Five isolates frequently isolated from the clean rooms of an aseptic pharmaceutical production workshop were selected based on their colony and cell morphology characteristics. Their physiological and biochemical properties, as well as partial 16S rDNA sequences, were analyzed. Results showed that all the five isolates belong to Gram positive bacteria, of which three were Staphylococcus, one Microbacterium and one Bacillus species. Sensitivity tests for these bacteria isolates to 3 disinfectants showed that isolate F

SUBMITTER: Wu GF 

PROVIDER: S-EPMC1963433 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature