An ectromelia virus profilin homolog interacts with cellular tropomyosin and viral A-type inclusion protein.

ABSTRACT:

Background

Profilins are critical to cytoskeletal dynamics in eukaryotes; however, little is known about their viral counterparts. In this study, a poxviral profilin homolog, ectromelia virus strain Moscow gene 141 (ECTV-PH), was investigated by a variety of experimental and bioinformatics techniques to characterize its interactions with cellular and viral proteins.

Results

Profilin-like proteins are encoded by all orthopoxviruses sequenced to date, and share over 90% amino acid (aa) identity. Sequence comparisons show highest similarity to mammalian type 1 profilins; however, a conserved 3 aa deletion in mammalian type 3 and poxviral profilins suggests that these homologs may be more closely related. Structural analysis shows that ECTV-PH can be successfully modelled onto b

SUBMITTER: Butler-Cole C 

PROVIDER: S-EPMC1964790 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature