A yeast model for target-primed (non-LTR) retrotransposition.

ABSTRACT:

Background

Target-primed (non-LTR) retrotransposons, such as the human L1 element, are mobile genetic elements found in many eukaryotic genomes. They are often present in large numbers and their retrotransposition can cause mutations and genomic rearrangements. Despite their importance, many aspects of their replication are not well understood.

Results

We have developed a yeast model system for studying target-primed retrotransposons. This system uses the Zorro3 element from Candida albicans. A cloned copy of Zorro3, tagged with a retrotransposition indicator gene, retrotransposes at a high frequency when introduced into an appropriate C. albicans host strain. Retrotransposed copies of the tagged element exhibit similar features to the native copies, indicating that the natu

SUBMITTER: Goodwin TJ 

PROVIDER: S-EPMC1965478 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature