Gene expression of transporters and phase I/II metabolic enzymes in murine small intestine during fasting.

ABSTRACT:

Background

Fasting has dramatic effects on small intestinal transport function. However, little is known on expression of intestinal transport and phase I/II metabolism genes during fasting and the role the fatty acid-activated transcription factor PPARalpha may play herein. We therefore investigated the effects of fasting on expression of these genes using Affymetrix GeneChip MOE430A arrays and quantitative RT-PCR.

Results

After 24 hours of fasting, expression levels of 33 of the 253 analyzed transporter and phase I/II metabolism genes were changed. Upregulated genes were involved in transport of energy-yielding molecules in processes such as glycogenolysis (G6pt1) and mitochondrial and peroxisomal oxidation of fatty acids (Cact, Mrs3/4, Fatp2, Cyp4a10, Cyp4b1). Other induc

SUBMITTER: van den Bosch HM 

PROVIDER: S-EPMC1971072 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature