In vivo functional discrimination between plant thioredoxins by heterologous expression in the yeast Saccharomyces cerevisiae.

ABSTRACT: Whereas vertebrates possess only two thioredoxin genes, higher plants present a much greater diversity of thioredoxins. For example, Arabidopsis thaliana has five cytoplasmic thioredoxins (type h) and at least as many chloroplastic thioredoxins. The abundance of plant thioredoxins leads to the question whether the various plant thioredoxins play a similar role or have specific functions. Because most of these proteins display very similar activities on artificial or biological substrates in vitro, we developed an in vivo approach to answer this question. The disruption of both of the two Saccharomyces cerevisiae thioredoxin genes leads to pleiotropic effects including methionine auxotrophy, H2O2 hypersensitivity, altered cell cycle characteristics, and a limited ability to use methionine s

SUBMITTER: Mouaheb N 

PROVIDER: S-EPMC19738 | biostudies-literature | 1998 Mar

REPOSITORIES: biostudies-literature