Comparative genomics of Bordetella pertussis reveals progressive gene loss in Finnish strains.

ABSTRACT:

Background

Bordetella pertussis is a gram-negative bacterium that infects the human respiratory tract and causes pertussis or whooping cough. The disease has resurged in many countries including Finland where the whole-cell pertussis vaccine has been used for more than 50 years. Antigenic divergence has been observed between vaccine strains and clinical isolates in Finland. To better understand genome evolution in B. pertussis circulating in the immunized population, we developed an oligonucleotide-based microarray for comparative genomic analysis of Finnish strains isolated during the period of 50 years.

Methodology/principal findings

The microarray consisted of 3,582 oligonucleotides (70-mer) and covered 94% of 3,816 ORFs of Tohama I, the strain of which the genome has bee

SUBMITTER: Heikkinen E 

PROVIDER: S-EPMC1975675 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature