Magnetic microposts as an approach to apply forces to living cells.

ABSTRACT: Cells respond to mechanical forces whether applied externally or generated internally via the cytoskeleton. To study the cellular response to forces separately, we applied external forces to cells via microfabricated magnetic posts containing cobalt nanowires interspersed among an array of elastomeric posts, which acted as independent sensors to cellular traction forces. A magnetic field induced torque in the nanowires, which deflected the magnetic posts and imparted force to individual adhesions of cells attached to the array. Using this system, we examined the cellular reaction to applied forces and found that applying a step force led to an increase in local focal adhesion size at the site of application but not at nearby nonmagnetic posts. Focal adhesion recruitment was enhanced furthe

SUBMITTER: Sniadecki NJ 

PROVIDER: S-EPMC1976246 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature