Experimental comparison and evaluation of the Affymetrix exon and U133Plus2 GeneChip arrays.

ABSTRACT:

Background

Affymetrix exon arrays offer scientists the only solution for exon-level expression profiling at the whole-genome scale on a single array. These arrays feature a new chip design with no mismatch probes and a radically new random primed protocol to generate sense DNA targets along the entire length of the transcript. In addition to these changes, a limited number of validating experiments and virtually no experimental data to rigorously address the comparability of all-exon arrays with conventional 3'-arrays result in a natural reluctance to replace conventional expression arrays with the new all-exon platform.

Methodology

Using commercially available Affymetrix arrays, we assess the performance of the Human Exon 1.0 ST (HuEx) and U133 Plus 2.0 (U133Plus2) platform

SUBMITTER: Abdueva D 

PROVIDER: S-EPMC1976394 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature