Clusterin expression can be modulated by changes in TCF1-mediated Wnt signaling.

ABSTRACT:

Background

Clusterin (CLU) is an enigmatic molecule associated with various physiological processes and disease states. Different modes of cellular stress lead to increased CLU levels, and additionally numerous growth factors and cytokines affect the expression of the CLU gene. APC and c-MYC, both intimately linked to the Wnt signaling pathway have previously been shown to influence CLU levels, and we therefore investigated if changes in Wnt signaling activity in vitro could regulate the expression of one, or more, of several CLU mRNA and protein variants.

Results

Over-expression of the cytoplasmic domain of E-cadherin tagged with GFP was used to abrogate Wnt signaling activity in LS174T and HCT116 colon carcinoma cells. This fusion construct sequestered signaling competent

SUBMITTER: Schepeler T 

PROVIDER: S-EPMC1976611 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature