Brucella suis urease encoded by ure1 but not ure2 is necessary for intestinal infection of BALB/c mice.

ABSTRACT:

Background

In prokaryotes, the ureases are multi-subunit, nickel-containing enzymes that catalyze the hydrolysis of urea to carbon dioxide and ammonia. The Brucella genomes contain two urease operons designated as ure1 and ure2. We investigated the role of the two Brucella suis urease operons on the infection, intracellular persistence, growth, and resistance to low-pH killing.

Results

The deduced amino acid sequence of urease-alpha subunits of operons-1 and -2 exhibited substantial identity with the structural ureases of alpha- and beta-proteobacteria, Gram-positive and Gram-negative bacteria, fungi, and higher plants. Four ure deficient strains were generated by deleting one or more of the genes encoding urease subunits of B. suis strain 1330 by allelic exchange: strain 13

SUBMITTER: Bandara AB 

PROVIDER: S-EPMC1983905 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature