Inhibition of histone deacetylase activity induces developmental plasticity in oligodendrocyte precursor cells.

ABSTRACT: Recently, it was demonstrated that lineage-committed oligodendrocyte precursor cells (OPCs) can be converted to multipotent neural stem-like cells, capable of generating both neurons and glia after exposure to bone morphogenetic proteins. In an effort to understand and control the developmental plasticity of OPCs, we developed a high-throughput screen to identify novel chemical inducers of OPC reprogramming. Using this system, we discovered that inhibition of histone deacetylase (HDAC) activity in OPCs acts as a priming event in the induction of developmental plasticity, thereby expanding the differentiation potential to include the neuronal lineage. This conversion was found to be mediated, in part, through reactivation of sox2 and was highly reproducible at the clonal level. Further, gen

SUBMITTER: Lyssiotis CA 

PROVIDER: S-EPMC1986599 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature