An expanded set of amino acid analogs for the ribosomal translation of unnatural peptides.

ABSTRACT:

Background

The application of in vitro translation to the synthesis of unnatural peptides may allow the production of extremely large libraries of highly modified peptides, which are a potential source of lead compounds in the search for new pharmaceutical agents. The specificity of the translation apparatus, however, limits the diversity of unnatural amino acids that can be incorporated into peptides by ribosomal translation. We have previously shown that over 90 unnatural amino acids can be enzymatically loaded onto tRNA.

Methodology/principal findings

We have now used a competition assay to assess the efficiency of tRNA-aminoacylation of these analogs. We have also used a series of peptide translation assays to measure the efficiency with which these analogs are incorpora

SUBMITTER: Hartman MC 

PROVIDER: S-EPMC1989143 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature