Diffusion-ordered nuclear magnetic resonance spectroscopy for analysis of DNA secondary structural elements.

ABSTRACT: Structure determination of secondary DNA structural elements, such as G-quadruplexes, gains an increasing importance as fundamental physiological roles are being associated with the formation of such structures in vivo. A truncated native DNA sequence generally requires further optimization to obtain a candidate with desired nuclear magnetic resonance (NMR) properties for structural analysis in solution. The optimum sequence is expected to form one dominant, stable molecular entity in solution with well-resolved NMR peaks. However, DNA sequences are prone to form structures composed of one, two, three, or four strands depending on sequence and solution conditions. The thorough characterization of the molecularity (stoichiometry and molecular weight) and appropriate solution conditions for

SUBMITTER: Ambrus A 

PROVIDER: S-EPMC1993845 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature