Enantioselective transacetylation of (R,S)-beta-citronellol by propanol rinsed immobilized Rhizomucor miehei lipase.

ABSTRACT:

Background

Use of enzymes in low water media is now widely used for synthesis and kinetic resolution of organic compounds. The frequently used enzyme form is the freeze-dried powders. It has been shown earlier that removal of water molecules from enzyme by rinsing with n-propanol gives preparation (PREP) which show higher activity in low water media. The present work evaluates PREP of the lipase (from Rhizomucor miehei) for kinetic resolution of (R,S)-beta-citronellol. The acylating agent was vinyl acetate and the reaction was carried out in solvent free media.

Results

The PREP, with 0.75% (v/v, reaction media) water, was indeed found to be more efficient and gave 95% conversion to the ester. Using this PREP, with no added water, 90% ee for (R)-(+)-beta-citronellyl acetate a

SUBMITTER: Majumder AB 

PROVIDER: S-EPMC1994055 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature