A minimal peach type II chlorophyll a/b-binding protein promoter retains tissue-specificity and light regulation in tomato.

ABSTRACT:

Background

Promoters with tissue-specificity are desirable to drive expression of transgenes in crops to avoid accumulation of foreign proteins in edible tissues/organs. Several photosynthetic promoters have been shown to be strong regulators of expression of transgenes in light-responsive tissues and would be good candidates for leaf and immature fruit tissue-specificity, if expression in the mature fruit were minimized.

Results

A minimal peach chlorophyll a/b-binding protein gene (Lhcb2*Pp1) promoter (Cab19) was isolated and fused to an uidA (beta-glucuronidase [GUS]) gene containing the PIV2 intron. A control vector carrying an enhanced mas35S CaMV promoter fused to uidA was also constructed. Two different orientations of the Cab19::GUS fusion relative to the left T-DNA b

SUBMITTER: Bassett CL 

PROVIDER: S-EPMC1994676 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature