Quantitative traits for the tail suspension test: automation, optimization, and BXD RI mapping.

ABSTRACT: Immobility in the tail suspension test (TST) is considered a model of despair in a stressful situation, and acute treatment with antidepressants reduces immobility. Inbred strains of mouse exhibit widely differing baseline levels of immobility in the TST and several quantitative trait loci (QTLs) have been nominated. The labor of manual scoring and various scoring criteria make obtaining robust data and comparisons across different laboratories problematic. Several studies have validated strain gauge and video analysis methods by comparison with manual scoring. We set out to find objective criteria for automated scoring parameters that maximize the biological information obtained, using a video tracking system on tapes of tail suspension tests of 24 lines of the BXD recombinant inbred pane

SUBMITTER: Lad HV 

PROVIDER: S-EPMC1998875 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature