Global transcriptional responses to cisplatin in Dictyostelium discoideum identify potential drug targets.

ABSTRACT: Dictyostelium discoideum is a useful model for studying mechanisms of cisplatin drug sensitivity. Our previous findings, that mutations in sphingolipid metabolism genes confer cisplatin resistance in D. discoideum and in human cells, raised interest in the resistance mechanisms and their implications for cisplatin chemotherapy. Here we used expression microarrays to monitor physiological changes and to identify pathways that are affected by cisplatin treatment of D. discoideum. We found >400 genes whose regulation was altered by cisplatin treatment of wild-type cells, including groups of genes that participate in cell proliferation and in nucleotide and protein metabolism, showing that the cisplatin response is orderly and multifaceted. Transcriptional profiling of two isogenic cisplatin-r

SUBMITTER: Van Driessche N 

PROVIDER: S-EPMC2000517 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature