Identification of novel proteins affected by rotenone in mitochondria of dopaminergic cells.

ABSTRACT:

Background

Many studies have shown that mitochondrial dysfunction, complex I inhibition in particular, is involved in the pathogenesis of Parkinson's disease (PD). Rotenone, a specific inhibitor of mitochondrial complex I, has been shown to produce neurodegeneration in rats as well as in many cellular models that closely resemble PD. However, the mechanisms through which complex I dysfunction might produce neurotoxicity are as yet unknown. A comprehensive analysis of the mitochondrial protein expression profile affected by rotenone can provide important insight into the role of mitochondrial dysfunction in PD.

Results

Here, we present our findings using a recently developed proteomic technology called SILAC (stable isotope labeling by amino acids in cell culture) combined wi

SUBMITTER: Jin J 

PROVIDER: S-EPMC2000881 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature