Identification of mouse hepatitis coronavirus A59 nucleocapsid protein phosphorylation sites.

ABSTRACT: The coronavirus nucleocapsid (N) is a multifunctional phosphoprotein that encapsidates the genomic RNA into a helical nucleocapsid within the mature virion. The protein also plays roles in viral RNA transcription and/or replication and possibly viral mRNA translation. Phosphorylation is one of the most common post-translation modifications that plays important regulatory roles in modulating protein functions. It has been speculated for sometime that phosphorylation could play an important role in regulation of coronavirus N protein functions. As a first step toward positioning to address this we have identified the amino acids that are phosphorylated on the mouse hepatitis coronavirus (MHV) A59 N protein. High performance liquid chromatography coupled with electrospray ionization tandem ma

SUBMITTER: White TC 

PROVIDER: S-EPMC2001268 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature