The dynamics of the roo transposable element in mutation-accumulation lines and segregating populations of Drosophila melanogaster.

ABSTRACT: We estimated the number of copies for the long terminal repeat (LTR) retrotransposable element roo in a set of long-standing Drosophila melanogaster mutation-accumulation full-sib lines and in two large laboratory populations maintained with effective population size approximately 500, all of them derived from the same isogenic origin. Estimates were based on real-time quantitative PCR and in situ hybridization. Considering previous estimates of roo copy numbers obtained at earlier stages of the experiment, the results imply a strong acceleration of the insertion rate in the accumulation lines. The detected acceleration is consistent with a model where only one (maybe a few) of the approximately 70 roo copies in the ancestral isogenic genome was active and each active copy caused new inser

SUBMITTER: Papaceit M 

PROVIDER: S-EPMC2013678 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature