Toward a systems biology of mouse inner ear organogenesis: gene expression pathways, patterns and network analysis.

ABSTRACT: We describe the most comprehensive study to date on gene expression during mouse inner ear (IE) organogenesis. Samples were microdissected from mouse embryos at E9-E15 in half-day intervals, a period that spans all of IE organogenesis. These included separate dissections of all discernible IE substructures such as the cochlea, utricle, and saccule. All samples were analyzed on high density expression microarrays under strict statistical filters. Extensive confirmatory tests were performed, including RNA in situ hybridizations. More than 5000 genes significantly varied in expression according to developmental stage, tissue, or both and defined 28 distinct expression patterns. For example, upregulation of 315 genes provided a clear-cut "signature" of early events in IE specification. Additio

SUBMITTER: Sajan SA 

PROVIDER: S-EPMC2013721 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature