Using gene chips to identify organ-specific, smooth muscle responses to experimental diabetes: potential applications to urological diseases.

ABSTRACT:

Objective

To identify early diabetes-related alterations in gene expression in bladder and erectile tissue that would provide novel diagnostic and therapeutic treatment targets to prevent, delay or ameliorate the ensuing bladder and erectile dysfunction.

Materials and methods

The RG-U34A rat GeneChip (Affymetrix Inc., Sunnyvale, CA, USA) oligonucleotide microarray (containing approximately 8799 genes) was used to evaluate gene expression in corporal and male bladder tissue excised from rats 1 week after confirmation of a diabetic state, but before demonstrable changes in organ function in vivo. A conservative analytical approach was used to detect alterations in gene expression, and gene ontology (GO) classifications were used to identify biological themes/pathways involved

SUBMITTER: Hipp JD 

PROVIDER: S-EPMC2013735 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature