Proteomic analyses of retina of excitatory amino acid carrier 1 deficient mice.

ABSTRACT:

Background

Excitatory amino acid carrier 1 (EAAC1) is a glutamate transporter found in neuronal tissues and is extensively expressed in the retina. EAAC1 plays a role in a variety of neural functions, but its biological functions in the retina has not been fully determined. The purpose of this study was to identify proteins regulated by EAAC1 in the retina of mice. To accomplish this, we used a proteomics-based approach to identify proteins that are up- or down-regulated in EAAC1-deficient (EAAC1-/-) mice.

Results

Proteomic analyses and two-dimensional gel electorphoresis were performed on the retina of EAAC1-/- mice, and the results were compared to that of wild type mice. The protein spots showing significant differences were selected for identification by mass spectrometr

SUBMITTER: Okumichi H 

PROVIDER: S-EPMC2014740 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature