Genome-wide expression profiling of the Arabidopsis female gametophyte identifies families of small, secreted proteins.

ABSTRACT: The female gametophyte of flowering plants, the embryo sac, develops within the diploid (sporophytic) tissue of the ovule. While embryo sac-expressed genes are known to be required at multiple stages of the fertilization process, the set of embryo sac-expressed genes has remained poorly defined. In particular, the set of genes responsible for mediating intracellular communication between the embryo sac and the male gametophyte, the pollen grain, is unknown. We used high-throughput cDNA sequencing and whole-genome tiling arrays to compare gene expression in wild-type ovules to that in dif1 ovules, which entirely lack embryo sacs, and myb98 ovules, which are impaired in pollen tube attraction. We identified nearly 400 genes that are downregulated in dif1 ovules. Seventy-eight percent of thes

SUBMITTER: Jones-Rhoades MW 

PROVIDER: S-EPMC2014789 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature