Microarray analysis in the archaeon Halobacterium salinarum strain R1.

ABSTRACT:

Background

Phototrophy of the extremely halophilic archaeon Halobacterium salinarum was explored for decades. The research was mainly focused on the expression of bacteriorhodopsin and its functional properties. In contrast, less is known about genome wide transcriptional changes and their impact on the physiological adaptation to phototrophy. The tool of choice to record transcriptional profiles is the DNA microarray technique. However, the technique is still rarely used for transcriptome analysis in archaea.

Methodology/principal findings

We developed a whole-genome DNA microarray based on our sequence data of the Hbt. salinarum strain R1 genome. The potential of our tool is exemplified by the comparison of cells growing under aerobic and phototrophic conditions, respectiv

SUBMITTER: Twellmeyer J 

PROVIDER: S-EPMC2020435 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature