Genome-wide polysomal analysis of a yeast strain with mutated ribosomal protein S9.

ABSTRACT:

Background

The yeast ribosomal protein S9 (S9) is located at the entrance tunnel of the mRNA into the ribosome. It is known to play a role in accurate decoding and its bacterial homolog (S4) has recently been shown to be involved in opening RNA duplexes. Here we examined the effects of changing the C terminus of S9, which is rich in acidic amino acids and extends out of the ribosome surface.

Results

We performed a genome-wide analysis to reveal effects at the transcription and translation levels of all yeast genes. While negligible relative changes were observed in steady-state mRNA levels, a significant number of mRNAs appeared to have altered ribosomal density. Notably, 40% of the genes having reliable signals changed their ribosomal association by more than one ribosome.

SUBMITTER: Pnueli L 

PROVIDER: S-EPMC2020489 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature