Non-coding antisense transcription detected by conventional and single-stranded cDNA microarray.

ABSTRACT:

Background

Recent studies revealed that many mammalian protein-coding genes also transcribe their complementary strands. This phenomenon raises questions regarding the validity of data obtained from double-stranded cDNA microarrays since hybridization to both strands may occur. Here, we wanted to analyze experimentally the incidence of antisense transcription in human cells and to estimate their influence on protein coding expression patterns obtained by double-stranded microarrays. Therefore, we profiled transcription of sense and antisense independently by using strand-specific cDNA microarrays.

Results

Up to 88% of expressed protein coding loci displayed concurrent expression from the complementary strand. Antisense transcription is cell specific and showed a strong tende

SUBMITTER: Vallon-Christersson J 

PROVIDER: S-EPMC2020490 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature