Manduca sexta prophenoloxidase activating proteinase-1 (PAP-1) gene: organization, expression, and regulation by immune and hormonal signals.

ABSTRACT: Insect phenoloxidase (PO) participates in melanotic encapsulation, wound healing, and cuticle sclerotization. It is converted from prophenoloxidase (proPO) by a proPO-activating proteinase (PAP). Manduca sexta PAP-1, the final component of a serine proteinase cascade, cleaves proPO to generate active PO. In an effort to understand the transcriptional regulation, we isolated a genomic clone of the PAP-1 gene, determined its nucleotide sequence, and elucidated its exon-intron organization. Computer analysis revealed several immune and hormone responsive elements in the upstream region. Southern blot analysis suggested that the M. sexta genome contains a single copy of PAP-1 gene. Reverse transcription-polymerase chain reaction showed that PAP-1 was constitutively expressed in fat body, trach

SUBMITTER: Zou Z 

PROVIDER: S-EPMC2020815 | biostudies-literature | 2005 Jun

REPOSITORIES: biostudies-literature