Predicting active site residue annotations in the Pfam database.

ABSTRACT:

Background

Approximately 5% of Pfam families are enzymatic, but only a small fraction of the sequences within these families (<0.5%) have had the residues responsible for catalysis determined. To increase the active site annotations in the Pfam database, we have developed a strict set of rules, chosen to reduce the rate of false positives, which enable the transfer of experimentally determined active site residue data to other sequences within the same Pfam family.

Description

We have created a large database of predicted active site residues. On comparing our active site predictions to those found in UniProtKB, Catalytic Site Atlas, PROSITE and MEROPS we find that we make many novel predictions. On investigating the small subset of predictions made by these databases that a

SUBMITTER: Mistry J 

PROVIDER: S-EPMC2025603 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature