Differential gene expression and Hog1 interaction with osmoresponsive genes in the extremely halotolerant black yeast Hortaea werneckii.

ABSTRACT:

Background

Fluctuations in external salinity force eukaryotic cells to respond by changes in the gene expression of proteins acting in protective biochemical processes, thus counteracting the changing osmotic pressure. The high-osmolarity glycerol (HOG) signaling pathway is essential for the efficient up-regulation of the osmoresponsive genes. In this study, the differential gene expression of the extremely halotolerant black yeast Hortaea werneckii was explored. Furthermore, the interaction of mitogen-activated protein kinase HwHog1 and RNA polymerase II with the chromatin in cells adapted to an extremely hypersaline environment was analyzed.

Results

A cDNA subtraction library was constructed for H. werneckii, adapted to moderate salinity or an extremely hypersaline environ

SUBMITTER: Vaupotic T 

PROVIDER: S-EPMC2034391 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature