Low rate of replication fork progression lengthens the replication timing of a locus containing an early firing origin.

ABSTRACT: Invariance of temporal order of genome replication in eukaryotic cells and its correlation with gene activity has been well-documented. However, recent data suggest a relax control of replication timing. To evaluate replication schedule accuracy, we detailed the replicational organization of the developmentally regulated php locus that we previously found to be lately replicated, even though php gene is highly transcribed in naturally synchronous plasmodia of Physarum. Unexpectedly, bi-dimensional agarose gel electrophoreses of DNA samples prepared at specific time points of S phase showed that replication of the locus actually begins at the onset of S phase but it proceeds through the first half of S phase, so that complete replication of php-containing DNA fragments occurs in late S phas

SUBMITTER: Benard M 

PROVIDER: S-EPMC2034475 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature