In vivo transcriptional targeting into the retinal vasculature using recombinant baculovirus carrying the human flt-1 promoter.

ABSTRACT:

Background

Endothelial cells are a target for gene therapy because they are implicated in a number of vascular diseases. Recombinant baculovirus have emerged as novel gene delivery vectors. However, there is no information available concerning the use of endothelial-specific promoters in the context of the baculovirus genome. In the present study, we have generated a recombinant baculovirus containing the human flt-1 promoter (BacFLT-GFP) driving the expression of the green fluorescent protein. Transcriptional gene targeting was analyzed in vitro in different mammalian cell lines and in vivo in adult rat retinal vasculature.

Results

BacFLT-GFP evoked the highest levels of expression in the endothelial cell line BUVEC-E6E7-1, similar to those reached by recombinant baculoviru

SUBMITTER: Luz-Madrigal A 

PROVIDER: S-EPMC2034561 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature