Noncoding transcription controls downstream promoters to regulate T-cell receptor alpha recombination.

ABSTRACT: The T early alpha (TEA) promoter in the murine Tcra locus generates noncoding transcripts that extend across the 65 kb Jalpha array. Here, we have analyzed the significance of TEA transcription for Tcra locus regulation through the targeted introduction of a transcription terminator downstream of the TEA promoter. We demonstrate that noncoding transcription driven by this single promoter can instruct both positively and negatively the activity of downstream Jalpha promoters, and can similarly instruct alterations in Jalpha chromatin structure and Jalpha recombination. TEA transcription activates promoters associated with relatively proximal Jalpha segments and stimulates histone acetylation, histone methylation and chromatin accessibility in this region. In contrast, at more distal locatio

SUBMITTER: Abarrategui I 

PROVIDER: S-EPMC2034674 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature