Significant and systematic expression differentiation in long-lived yeast strains.

ABSTRACT:

Background

Recent studies suggest that the regulation of longevity may be partially conserved in many eukaryotes ranging from yeast to mammals. The three yeast mutants sch9Delta, ras2Delta, tor1Delta show extended chronological life span up to three folds. Our aim is to dissect the mechanisms that lead to the yeast life span extension.

Methodology/principal findings

We obtain gene expression profiles of sch9Delta, ras2Delta, tor1Delta as well as that for a wild type at day 2.5 in SDC medium using Affymetrix Yeast2.0 arrays. To accurately estimate the expression differentiation between the wild type and the long-lived mutants, we use sub-array normalization followed by a variant of the median-polishing summarization. The results are validated by the probe sets of S. pombe on

SUBMITTER: Cheng C 

PROVIDER: S-EPMC2039703 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature