Inactivation of NMD increases viability of sup45 nonsense mutants in Saccharomyces cerevisiae.

ABSTRACT:

Background

The nonsense-mediated mRNA decay (NMD) pathway promotes the rapid degradation of mRNAs containing premature termination codons (PTCs). In yeast Saccharomyces cerevisiae, the activity of the NMD pathway depends on the recognition of the PTC by the translational machinery. Translation termination factors eRF1 (Sup45) and eRF3 (Sup35) participate not only in the last step of protein synthesis but also in mRNA degradation and translation initiation via interaction with such proteins as Pab1, Upf1, Upf2 and Upf3.

Results

In this work we have used previously isolated sup45 mutants of S. cerevisiae to characterize degradation of aberrant mRNA in conditions when translation termination is impaired. We have sequenced his7-1, lys9-A21 and trp1-289 alleles which are frequent

SUBMITTER: Chabelskaya S 

PROVIDER: S-EPMC2039749 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature