Serum protein profiling to identify high-risk neuroblastoma: preclinical relevance of blood-based biomarkers.

ABSTRACT:

Introduction

Development of early detection assays for advanced stage neuroblastoma (NB) remains elusive. We have previously shown that serum protein profiling technologies can differentiate healthy from NB children. As various sources of patient related bias exist in serum proteins, we hypothesized a well controlled animal model may provide a better method to identify tumor blood-based markers during NB progression.

Methods

Tumors were induced in the left kidneys of nude mice by the injection of cultured human NB cells (10(6)). Sera were collected from control and tumor-bearing mice at 2, 4, and 6 wk. Albumin-depleted sera were subjected to comparative proteomic profiling using 2D gel electrophoresis. Paired samples at each time point were analyzed and differentially expres

SUBMITTER: Sandoval JA 

PROVIDER: S-EPMC2040037 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature