In silico characterization of immunogenic epitopes presented by HLA-Cw*0401.

ABSTRACT:

Background

HLA-C locus products are poorly understood in part due to their low expression at the cell surface. Recent data indicate that these molecules serve as major restriction elements for human immunodeficiency virus type 1 (HIV-1) cytotoxic T lymphocyte (CTL) epitopes. We report here a structure-based technique for the prediction of peptides binding to Cw*0401. The models were rigorously trained, tested and validated using experimentally verified Cw*0401 binding and non-binding peptides obtained from biochemical studies. A new scoring scheme facilitates the identification of immunological hot spots within antigens, based on the sum of predicted binding energies of the top four binders within a window of 30 amino acids.

Results

High predictivity is achieved when tested

SUBMITTER: Tong JC 

PROVIDER: S-EPMC2040137 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature