A method for improving SELDI-TOF mass spectrometry data quality.

ABSTRACT:

Background

Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) is a powerful tool for rapidly generating high-throughput protein profiles from a large number of samples. However, the events that occur between the first and last sample run are likely to introduce technical variation in the results.

Methods

We fractionated and analyzed quality control and investigational serum samples on 3 Protein Chips and used statistical methods to identify poor-quality spectra and to identify and reduce technical variation.

Results

Using diagnostic plots, we were able to visually depict all spectra and to identify and remove those that were of poor quality. We detected a technical variation associated with when the samples were run (referred

SUBMITTER: Whistler T 

PROVIDER: S-EPMC2040139 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature