The LARK RNA-binding protein selectively regulates the circadian eclosion rhythm by controlling E74 protein expression.

ABSTRACT: Despite substantial progress in defining central components of the circadian pacemaker, the output pathways coupling the clock to rhythmic physiological events remain elusive. We previously showed that LARK is a Drosophila RNA-binding protein which functions downstream of the clock to mediate behavioral outputs. To better understand the roles of LARK in the circadian system, we sought to identify RNA molecules associated with it, in vivo, using a three-part strategy to (1) capture RNA ligands by immunoprecipitation, (2) visualize the captured RNAs using whole-genome microarrays, and (3) identify functionally relevant targets through genetic screens. We found that LARK is associated with a large number of RNAs, in vivo, consistent with its broad expression pattern. Overexpression of LARK in

SUBMITTER: Huang Y 

PROVIDER: S-EPMC2040218 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature