Small-molecule-mediated rescue of protein function by an inducible proteolytic shunt.

ABSTRACT: Controlling protein function through posttranslational manipulations has emerged as an attractive complementary technology to existing genetic systems. Often these methods involve developing pharmacological agents to probe protein function without the need to generate a unique compound for each protein family. One common strategy uses small molecules that act as chemical inducers of dimerization by mediating the interaction of two proteins. Herein we report the use of a chemical inducer of dimerization for the development of a posttranslational technology for the manipulation of protein function. This system, split ubiquitin for the rescue of function (SURF), places the complementation of genetically split ubiquitin under the control of rapamycin-induced dimerization of FK506-binding prote

SUBMITTER: Pratt MR 

PROVIDER: S-EPMC2040878 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature