FASTDXL: a generalized screen to trap disulfide-stabilized complexes for use in structural studies.

ABSTRACT: Structural studies of macromolecular complexes have produced extraordinary insights into a wide variety of biological processes. Unfortunately, as structural biologists pursue larger and more challenging assemblies, weakly stable and/or nonspecific interactions can become significant roadblocks to structure determination. We have developed a rapid and effective pool-based screen, termed FASTDXL (focused array screening technique for disulfide X-linking), to produce and identify disulfide-stabilized protein-nucleic acid assemblies. A significant strength of FASTDXL is that it can take advantage of prior structural knowledge about molecular interactions, but does not necessarily rely upon it. A detailed application of the approach to the difficult problem of trapping a bacterial primase-ssDN

SUBMITTER: Corn JE 

PROVIDER: S-EPMC2041450 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature