A proteomic study of the HUPO Plasma Proteome Project's pilot samples using an accurate mass and time tag strategy.

ABSTRACT: Characterization of the human blood plasma proteome is critical to the discovery of routinely useful clinical biomarkers. We used an accurate mass and time (AMT) tag strategy with high-resolution mass accuracy cLC-FT-ICR MS to perform a global proteomic analysis of pilot study samples as part of the HUPO Plasma Proteome Project. HUPO reference serum and citrated plasma samples from African Americans, Asian Americans, and Caucasian Americans were analyzed, in addition to a Pacific Northwest National Laboratory reference serum and plasma. The AMT tag strategy allowed us to leverage two previously published "shotgun" proteomics experiments to perform global analyses on these samples in triplicate in less than 4 days total analysis time. A total of 722 (22% with multiple peptide identification

SUBMITTER: Adkins JN 

PROVIDER: S-EPMC2041806 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature